Implemented kill switches: 1
Kill Switch 1
Class
# Format:
# Write how you would classify them.
Toxins
| Name |
Number |
Comment |
| Bacteriophage 21 Lysis Cassette S and R and Rz (PVJ4) |
BBa_K124003 |
consists of Holin (S) and Endolysin (R) which is the actual toxin and Rz protein |
Antagonists
| Name |
Number |
Comment |
| RNA thermometer (ROSE) |
BBa_K115001 |
RNA that binds at 37 deg C as dimer to a complementary sequence on the DNA and inhibits transkription. Linearization at 42 deg C allows transkription |
Topology
Parts
| Name |
Number |
Comment |
| RNA thermometer (ROSE) |
BBa_K115001 |
RNA that binds at 37 deg C as dimer to a complementary sequence on the DNA and inhibits transkription. Linearization at 42 deg C allows transkription |
| T7 Promoter |
BBa_I712074 |
strong Promoter of Bacteriophage T7 |
| GFP |
BBa_E0040 |
Reporterprotein to determine proteine expression levels |
| Bacteriophage 21 Lysis Cassette S and R and Rz (PVJ4) |
BBa_K124003 |
consists of Holin (S) and Endolysin (R) and Rz protein |